abstract：:The objective of visual systems neuroscience has shifted over the past few years from determining the receptive fields of cells towards the understanding of higher level cognition in awake animals viewing natural stimuli. In experiments with awake animals it is important to control the relevant aspects of behavior. Mo...

journal_title：Journal of neuroscience methods

authors： Körding KP,Kayser C,Betsch BY,König P

更新日期：2001-09-30 00:00:00

abstract：:Neurophysiologists have been investigating the responses of neurons in the visual system for the past half-century using monkeys and cats that are anesthetized and paralyzed, with the non-blinking eyelids open for prolonged periods of time. Impermeable plastic contact lenses have been used to prevent dehydration of th...

journal_title：Journal of neuroscience methods

authors： Metha AB,Crane AM,Rylander HG 3rd,Thomsen SL,Albrecht DG

更新日期：2001-08-30 00:00:00

abstract：:For interpretation of microdialysis experiments in which compounds are applied retrodialysis, it is important to have information about the migration rate of the infused compounds. Here we describe a dual-probe microdialysis method that can be used to evaluate the penetration rate of the infused drug. The basic idea i...

journal_title：Journal of neuroscience methods

authors： Westerink BH,De Vries JB

更新日期：2001-08-15 00:00:00

abstract：:The LTP Program is a stimulation, acquisition and on-line analysis program for studying long-term potentiation (LTP), long-term depression (LTD), and stimulus-evoked synaptic responses in general. The program is freely available from the website: www.ltp-program.com. It is a 32-bit DOS program that runs on Windows 3/9...

journal_title：Journal of neuroscience methods

authors： Anderson WW,Collingridge GL

更新日期：2001-07-15 00:00:00

abstract：:We have developed a novel method to examine [3H]-noradrenaline and [14C]-glutamate release from the same sample of streptolysin-O (SLO) perforated rat cortical synaptosomes. Ca2+ -dependent [3H]-noradrenaline and [14C]-glutamate release was examined at different temperatures and was found to be greater at 30 degrees C...

journal_title：Journal of neuroscience methods

authors： McFadden SC,Bobich JA,Zheng Q

更新日期：2001-05-30 00:00:00

abstract：:The present study demonstrates that bipotential neural precursors isolated from an early developmental stage of the sheep embryo nervous system can be maintained in vitro in an undifferentiated state for a long period. These precursors multiplied under the action of epidermal growth factor and basic fibroblast growth ...

journal_title：Journal of neuroscience methods

authors： Duittoz AH,Hevor T

更新日期：2001-05-30 00:00:00

abstract：:Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...

journal_title：Journal of neuroscience methods

authors： Buffelli M,Busetto G,Cangiano A

更新日期：2001-04-30 00:00:00

abstract：:The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five ...

journal_title：Journal of neuroscience methods

authors： Land BR,Wyttenbach RA,Johnson BR

更新日期：2001-03-30 00:00:00

abstract：:Microdialysis is a useful tool for administering drugs into localized regions of brain tissue, but the diffusion of drugs from the probe has not been systematically examined. Lidocaine (10%) and tetrodotoxin (TTX, 10 microM), drugs typically used in neural inactivation studies, were infused through a microdialysis pro...

journal_title：Journal of neuroscience methods

authors： Boehnke SE,Rasmusson DD

更新日期：2001-02-15 00:00:00

abstract：:Amperometry is a very powerful technique for investigating the role(s) specific proteins play in exocytosis at the single-cell level. In this study, amperometry has been used to investigate possible changes in exocytosis at chromaffin cells isolated from coloboma and tottering mutant mice. Coloboma mice possess a dele...

journal_title：Journal of neuroscience methods

authors： Colliver TL,Hess EJ,Ewing AG

更新日期：2001-01-30 00:00:00

abstract：:A computer model of the hippocampal CA1 area, which receives synaptic inputs from CA3 neurons via the Schaffer collaterals, was constructed. Pyramidal cells (PC) and two types of interneurons were represented by compartmental models, and mechanisms of feed-forward inhibition (FFI) and recurrent inhibition were incorpo...

journal_title：Journal of neuroscience methods

authors： Sargsyan AR,Papatheodoropoulos C,Kostopoulos GK

更新日期：2001-01-15 00:00:00

abstract：:The cerebellar Purkinje neuron (PN) serves as an important model in studies of neuronal development in the mammalian central nervous system. Dissociated PN preparations maintained in an in-vitro environment with simplified cellular and biochemical conditions can facilitate molecular analyses of neuronal development. H...

journal_title：Journal of neuroscience methods

authors： Tabata T,Sawada S,Araki K,Bono Y,Furuya S,Kano M

更新日期：2000-12-15 00:00:00

abstract：:We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...

journal_title：Journal of neuroscience methods

authors： Vidulich L,Mitchell D

更新日期：2000-11-30 00:00:00

abstract：:Most techniques used for the study of the fiber connectivity in the central nervous system produce results which are visualized in the conventional light microscope or fluorescence microscope. Although in some cases this may be sufficient, often proof is necessary that fibers which enter a particular brain area indeed...

journal_title：Journal of neuroscience methods

authors： Van Haeften T,Wouterlood FG

更新日期：2000-11-15 00:00:00

abstract：:This study demonstrated that liposome-mediated transfection - lipofection - is suitable for delivering genes into astrocytes. By repeatedly lipofecting the same astrocyte cultures, a process we call multi-lipofection, the transfection efficiency of the beta-galactosidase (beta-gal) gene was improved from 2.6+/-0.6 to ...

journal_title：Journal of neuroscience methods

authors： Wu BY,Liu RY,So KL,Yu AC

更新日期：2000-10-30 00:00:00

abstract：:2,3,5-Triphenyltetrazolium chloride (TTC), a marker of mitochondrial enzyme activity, is widely used to assess the effects of cerebral ischaemia in vivo. In the present study, we characterised its utility as a simple rapid macrohistological measure of ischaemic damage in brain slices. Coronal rat corticostriatal slice...

journal_title：Journal of neuroscience methods

authors： Mathews KS,McLaughlin DP,Ziabari LH,Toner CC,Street PC,Hisgrove E,Bezzina EL,Stamford JA

更新日期：2000-10-15 00:00:00

abstract：:Using the novel mathematical technique known as wavelet analysis, a new method (WSC) is presented to sort spikes according to a decomposition of neural signals in the time-frequency space. The WSC method is implemented by a pyramidal algorithm that acts upon neural signals as a bank of quadrature mirror filters. This ...

journal_title：Journal of neuroscience methods

authors： Letelier JC,Weber PP

更新日期：2000-09-15 00:00:00

abstract：:This paper describes a computer program, named ASAP (audio sequencing and presentation), that runs on IBM PC compatible hardware with a Microsoft Windows operating system and has been specially designed for facilitating auditory neuroimaging studies. The program's main characteristics are: (1) the ability to generate ...

journal_title：Journal of neuroscience methods

authors： Kieckhefer E,Kanwal JS

更新日期：2000-08-15 00:00:00

abstract：:Prehensile behavior is a popular task in current research on human motor control. Most studies on reaching used stationary target objects and, therefore, most models do not address the challenges the motor system must respond to when reaching for moving objects. The machines used in earlier studies to produce object m...

journal_title：Journal of neuroscience methods

authors： Schenk T,Philipp J,Häussler A,Hauck A,Hermsdörfer J,Mai N

更新日期：2000-07-31 00:00:00

abstract：:This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10-15 microm), imprinting does ...

journal_title：Journal of neuroscience methods

authors： Thomson CE,Griffiths IR

更新日期：2000-07-31 00:00:00

abstract：:An opto-electronic system has been developed to measure movements of insect appendages. It is made from a mirror-lens and a linear position-sensitive photodiode. The design of the mirror-lens has been exploited to axially mount a high intensity halogen light source in front of the mirror-lens. The system monitors a re...

journal_title：Journal of neuroscience methods

authors： Hedwig B

更新日期：2000-07-31 00:00:00

abstract：:In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Im...

journal_title：Journal of neuroscience methods

authors： Zhu M,Klimek V,Haycock JW,Ordway GA

更新日期：2000-06-30 00:00:00

abstract：:We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it wa...

journal_title：Journal of neuroscience methods

authors： Verdú E,Rodríguez FJ,Gudiño-Cabrera G,Nieto-Sampedro M,Navarro X

更新日期：2000-06-30 00:00:00

abstract：:Motor neurons innervating tiny muscles in the antennal system of the cockroach, Periplaneta americana, were stained by two new approaches to backfilling motor axons and somata. Both methods are easier, and produce less leakage of dye, than conventional methods that rely on application of dye to the distal cut end of a...

journal_title：Journal of neuroscience methods

authors： Baba Y

更新日期：2000-06-01 00:00:00

abstract：:We examined topographic variations in nonlinear measures based on scalp voltages, which were generated by two simulated current dipoles each placed in a different hemisphere of a spherical volume conductor (three-shell model). Dipole dynamics were that of a three-torus and the x-component of the Lorenz-system and scal...

journal_title：Journal of neuroscience methods

authors： Fell J,Hauk O,Hinrichs H

更新日期：2000-05-15 00:00:00

abstract：:Intrinsic signal imaging (ISI) measures changes in light reflectance from the illuminated cortex (intrinsic signals or IS) attributed to various vascular and metabolic sources that, when using illumination in the 600 nm range, appear to co-localize with neuronal activity. Given the multiple sources contributing to the...

journal_title：Journal of neuroscience methods

authors： Chen-Bee CH,Polley DB,Brett-Green B,Prakash N,Kwon MC,Frostig RD

更新日期：2000-04-15 00:00:00

abstract：:We present a quantified imaging method that describes the cell column in mammalian cortex. The minicolumn is an ideal template with which to examine cortical organization because it is a basic unit of function, complete in itself, which interacts with adjacent and distance columns to form more complex levels of organi...

journal_title：Journal of neuroscience methods

authors： Buxhoeveden DP,Switala AE,Roy E,Casanova MF

更新日期：2000-04-01 00:00:00

abstract：:Studies of the neural control of movement often require or benefit from long-term recording of EMG activity from large numbers of muscles involved in a particular motor task. While chronic recording of EMG activity has been described in a number of previous monkey studies, the number of muscles recorded has been somew...

journal_title：Journal of neuroscience methods

authors： Park MC,Belhaj-Saïf A,Cheney PD

更新日期：2000-03-15 00:00:00

abstract：:This paper presents a technique for stimulating and recording from multiple intact spinal roots in the in vitro mudpuppy (Necturus maculatus) spinal cord-forearm preparation using fine wire electrodes, a modified intrafascicular electrode. We found that multiple spinal roots of the preparation could be implanted with ...

journal_title：Journal of neuroscience methods

authors： Yoshida K,Jovanović K,Stein RB

更新日期：2000-03-01 00:00:00

abstract：:Capsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximately 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately or 2 months later by...

journal_title：Journal of neuroscience methods

authors： Puigdellívol-Sánchez A,Prats-Galino A,Ruano-Gil D,Molander C

更新日期：2000-02-15 00:00:00

abstract：:We describe a method for estimating the average proportion of GABAergic terminal area relative to total nerve terminal area with confocal microscopy. Nerve terminal regions were identified with dual colour immunofluorescence on Vibratome sections with an antibody to synaptophysin (SYN), and GABAergic processes, includ...

journal_title：Journal of neuroscience methods

authors： Hiscock JJ,Murphy S,Willoughby JO

更新日期：2000-01-31 00:00:00

abstract：:Information concerning the location and distribution of presynaptic neurotransmitter release sites within anatomically labeled axons would be of value for a large number of studies in functional anatomy, development, and plasticity. Here we report a method for localizing presynaptic sites within identified arbors of i...

journal_title：Journal of neuroscience methods

authors： Silver MA,Stryker MP

更新日期：2000-01-15 00:00:00

abstract：:Principal components analysis (PCA) was used to define the linearly dependent factors underlying sensory information processing in the vibrissal sensory area of the ventral posterior medial (VPM) thalamus in eight awake rats. Ensembles of up to 23 single neurons were simultaneously recorded in this area, either during...

journal_title：Journal of neuroscience methods

authors： Chapin JK,Nicolelis MA

更新日期：1999-12-15 00:00:00

abstract：:The masking of antigens by aldehyde-containing fixatives or by paraffin embedding procedures is a problem for immunohistochemical studies. Enzymatic digestion, formic acid treatment, microwave heating and autoclave heating have been used to deal with this problem, with microwave heating-based antigen retrieval having ...

journal_title：Journal of neuroscience methods

authors： Jiao Y,Sun Z,Lee T,Fusco FR,Kimble TD,Meade CA,Cuthbertson S,Reiner A

更新日期：1999-11-15 00:00:00

abstract：:We describe a method for in vivo confocal fluorescence imaging of synaptic terminals and subsequent electron microscopic reconstructions of the same terminals. By iontophoretically applying lipophilic dye to nerve terminals at a single neuromuscular junction with a sharp microelectrode in living neonatal mice, we were...

journal_title：Journal of neuroscience methods

authors： Gan WB,Bishop DL,Turney SG,Lichtman JW

更新日期：1999-10-30 00:00:00

abstract：:The lability of brain myosin-V (BM-V) to aldehyde-fixation has hindered immunohistochemical (IH) studies of this actin-based motor. We show here that BM-V immunoreactivity (IR) can be retrieved from formalin-fixed, paraffin-embedded human tissue. BM-V IR was optimally retrieved by boiling 5 microm cerebellar tissue se...

journal_title：Journal of neuroscience methods

authors： Martins AR,Dias MM,Vasconcelos TM,Caldo H,Costa MC,Chimelli L,Larson RE

更新日期：1999-10-15 00:00:00

abstract：:Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices with excitation po...

journal_title：Journal of neuroscience methods

authors： Tan YP,Llano I,Hopt A,Würriehausen F,Neher E

更新日期：1999-10-15 00:00:00

abstract：:The immunotoxin 192 IgG-saporin has a high degree of selectivity for cholinergic neurons within the basal forebrain (CBF). Intracerebroventricular delivery of 192 IgG-saporin results in a diffuse and massive depletion of choline acetyltransferase (ChAT) activity in projections of the CBF, and non-selective loss of Pur...

journal_title：Journal of neuroscience methods

authors： Pizzo DP,Waite JJ,Thal LJ,Winkler J

更新日期：1999-09-15 00:00:00

abstract：:Evaluation of the amount of overall animal movement is important for investigations of motor control mechanisms in the central nervous system. We describe a new method to quantify overall free movements of an animal without any markers using a video camera and a personal computer equipped with a video-capture board. T...

journal_title：Journal of neuroscience methods

authors： Hashimoto T,Izawa Y,Yokoyama H,Kato T,Moriizumi T

更新日期：1999-09-15 00:00:00

abstract：:This study aimed at developing a laser-Doppler flowmetry (LDF) device suitable for long-term cortical cerebral blood flow (cCBF) measurement in awake, freely moving rats. The device included a flow probe adapter for permanent fixation to the skull bone and a connector that held the flow probe in the adapter in exactly...

journal_title：Journal of neuroscience methods

authors： Gu WG,Jiang W,Brännström T,Wester P

更新日期：1999-08-01 00:00:00